FAQs Page

  • Laboratory serology tests can be ordered online, over the phone by calling 844-441-8610 or via
    email [email protected].
  • Carefully package the garment or item to be tested in a paper bag or envelope.
  • On the outside of the paper bag or envelope, write your contact information, date you obtained
    the sample, order number that you received at checkout, and any other relevant information to
    the sample.
  • Please DO NOT use a plastic mailer or poly/plastic bag or wrapping around the garment, as this
    may cause the item to sweat and destroy the sample.
  • Place samples in a larger paper mailer or box and send it to SSDI, 2450 Hollywood Blvd. Suite
    700 Hollywood, Florida 33020 in envelope or paper bag.
  • Visit our page on “Handling and storage of a suspicious pair of underwear” and “packaging
    items” for more information and details on shipping your sample.
  • Once we receive your sample, we will have your results ready in 4-7 business days!
  • At home test kits (link to at home test kits) are designed for you to purchase so that you can do the testing yourself at home. We will ship you the kit(s) you ordered with the required tools and instructions for testing.
  • With the Laboratory Semen Sperm Saliva detection testing, you ship the sample to us, and we will perform a test for semen, sperm, saliva, and blood when necessary. We will send you a report containing the results once the testing is completed. The
    laboratory testing is recommended because PSA is only a presumptive test for semen and needs to be confirmed by another method. The only way to confirm the presence of semen is via microscopic examination which is done by our trained laboratory technicians during the laboratory testing process. Our laboratory technicians are also trained to look for signs of infidelity on samples and can give you a professional assessment beyond the scope of an at home test. 

We generally recommend the destructive Semen Sperm Saliva Detection testing to all of our customers. This test generally produces better results than the non- destructive semen, sperm, saliva detection test since the stain can be extracted directly from the map of the fibers. We recommend the laboratory tests over the at home tests because they provide comprehensive and conclusive results.

Yes, although it is rare, PSA has been experimentally documented in both male & female blood, urine, & saliva, female vaginal fluid, and of course male seminal fluid (semen minus sperm cells) so it is not a confirmatory test for semen or seminal fluid. The microscopic proof of sperm cells is the only way to confirm the presence of semen.

It is possible for sperm to survive the laundering process, though studies have shown that they tend to be very few in number. PSA proteins generally do not survive the laundering process and will generally produce negative results if the garment has been washed.

It takes the body about 48 hours (3 days max) to breakdown the PSA and 5-12 days to break down sperm cells as sperm cells remain in the body much longer than PSA. If the test results for PSA are positive, it may indicate that she has had intercourse with another male. If sperm cells are detected, it would be best to proceed with DNA testing to determine if you or another male are the DNA donor.

Yes, if the underwear is worn immediately after intercourse, the PSA can last for years and the sperm can last forever in the dried stain, as long as it is not washed or exposed to bacteria, UV light, or extreme heat.

In the case of vasectomized individuals, it is possible that the vasectomy was not complete, and they may still produce few sperm, or they may not. Oligospermic individuals produce a very small number of sperm. Azoospermic individuals do not produce sperm at all. In either case, seminal fluid is still produced. Semen is made up of sperm and seminal fluid. PSA is used as a presumptive test for the presence of seminal fluid because it is one of the major proteins found in seminal fluid. If an individual cannot produce sperm, or produces a very low number of sperm, the results may state that PSA is positive, but sperm is negative and therefore the results for semen would be negative as well.

While the majority of the samples that we receive for testing consist of male and female undergarments, we have also tested hundreds of non-clothing items such as used tissues, feminine products, shower drains, wood chips, carpet, condoms, pizza, hair, spit in baggies and cups, finger wiping on tissues, floors, combs and brushes, food wrappers, feminine pads and tampons, band aids, fistulas from medical procedures, bed linens, bed posts, wash cloths and towels, and toothbrushes. If you have any questions about an item, feel free to give us a call.

We can test samples of any age. There is data supporting 30-year-old samples testing positive for semen. If the underwear is worn immediately post-coital (immediately after intercourse), PSA will last for years and sperm will last forever in the dried stain unless laundered or exposed to bacteria, UV light, or extreme heat. Our test for human blood has also been proven to be accurate even on 31-year-old samples. In all cases, it is crucial to ensure proper handling and storage of the sample. Learn more information on sample storage and handling here

We recommend that you do not let the outcome of any home semen detection test result in a life changing situation (such as a divorce). If you confront your spouse before you have 100% conclusive results you could jeopardize your relationship. The best option would be to have our experienced laboratory technicians test your sample so you can be 100% confident in your results.

A black light can be helpful in locating biological stains for testing. No expert can look at a random set of stains and tell you which one is semen as many different biological fluids as well as even household cleaners will fluoresce under UV light. We use an expensive selectable multiband UV forensic lamp to fluoresce each article before the extraction is accomplished so that we obtain sampling from every stain.

Our At Home Semen Detection Kit for PSA tests Prostate specific Antigen (PSA). PSA is a protein that is found in large quantities in seminal fluid but is only a presumptive test for semen as semen consists of both PSA and sperm. Positive identification of sperm microscopically is the
only confirmatory test for semen. This means that the only way to confirm the presence of semen is to observe sperm microscopically. Additionally, PSA can be found in very small quantities in other bodily fluids, hence why it is only a presumptive test for semen. If you want to confirm the presence of semen, the best option is to send the sample into the laboratory for testing.

With every laboratory test, we offer a free case consultation to determine which test is right for you. The laboratory testing includes testing for Prostate specific antigen (PSA), a microscopic sperm assessment, a test for human saliva (HSA), and a test for human blood (HEM) when warranted, a laboratory report containing the test results, and free sample storage for one year if positive and 90 days if negative after testing is completed.

All tested samples are automatically stored for one year if positive and 90 days if negative (included with test). Additional sample storage can also be purchased. If you would like to have your article returned to you after testing, you can purchase article return here.

Hemoglobin (Hb) is one of the most abundant proteins in blood. It is the red blood pigment located in erythrocytes that transports oxygen and carbon dioxide throughout the body. Our test allows for the detection of hemoglobin on samples containing only 40ng/ml of human hemoglobin. Hemoglobin is used for the detection of blood in infidelity cases as a positive result may indicate indiscernible menstrual or vaginal bleeding from intercourse.

Vaginal discharge is the discharge of secretions from the cervical glands of the vagina and is normally clear or white in color. Vaginal discharge can be distributed in multiple locations within a pair of male underwear after intercourse. Unfortunately, there is no single test that can be done for the identification of vaginal fluid as it does not contain any characteristic proteins for identification.

Semen is a mixture that comes from secretions of the testes, seminal vesicles, prostate, and bulbourethral glands (Cowper’s glands) and is the medium of travel for sperm. Seminal plasma is the most prominent ingredient of human semen. Seminal plasma is a fluid that is secreted by the seminal vesicles and prostate gland, which make up the male reproductive organs. Theseminal plasma mediates the chemical function of the ejaculate. Semen’s purpose is to feed and protect the spermatozoa (sperm cell) during their long trip up to the female’s ova via acidic
conditions it encounters in the female vagina. Over 95% of the ejaculate volume originates from the sex accessory tissues and not from the testes, as is often mistakenly believed. A human male’s ejaculate volume is about 3 ml and ranges from 2 to 6 ml. Of the 3 ml of an ejaculate, a
very small proportion, 0.2 ml, originates from the Cowper’s gland and 0.5 ml from the prostate gland. The largest portion, approximately 2 ml, is secreted from the seminal vesicles and appears in the latter portion of the ejaculate volume.

PSA (Prostate- Specific Antigen) is a glycoprotein that is secreted into seminal fluid. Healthy fertile males generally secrete 1-6 ml of seminal fluid during ejaculation. PSA is one of the major proteins in seminal fluid with concentrations of 0.2 to 3.0 mg/ml. Because of its very high concentration, PSA has been used as a marker protein for seminal fluid in the forensics field. The amount of PSA is independent of sperm cells. It can be detected regardless of an individual’s sperm cells presence and can be detected in cases of vasectomized men. PSA is a very stable protein in its dried state and can be extracted from stains over 30 years old.

Sperm, also called sperm cell or spermatozoon, are the mature fertilizing gamete of a male organism. They usually consist of a round or cylindrical nucleated cell, a short neck, and a thin motile tail. Healthy fertile males generally secrete about 40 million sperms per ml of seminal fluid during ejaculation. Oligospermic individuals have a drastically lower amount of sperm cells and Azoospermic or vasectomized individuals generally do not produce sperm. Sperm is only found in semen. A positive identification of sperm is the only way to confirm the presence of semen.

HSA (human salivary amylase) is a protein that is a major component of saliva. The enzyme αamylase is found in particularly high concentrations in saliva and primarily serves to break down polysaccharides. A positive saliva identification in infidelity cases can indicate oral sex with or
without intercourse. We have many cases where the suspect is caught by positive saliva results when the semen detection test was negative. We have also had other cases including negative semen detections where human saliva is detected and proven by a DNA analysis to be from a
male (not the husband). We integrated saliva testing into our infidelity detection testing in 2007 and are the only private company that includes saliva detection as part of every semen detection test.

  • HSA (human salivary amylase) is a protein that is a major component of saliva. The enzyme αamylase is found in particularly high concentrations in saliva and primarily serves to break down polysaccharides. A positive saliva identification in infidelity cases can indicate oral sex with or without intercourse. We have many cases where the suspect is caught by positive saliva results when the semen detection test was negative. We have also had other cases including negative semen detections where human saliva is detected and proven by a DNA analysis to be from a male (not the husband). We integrated saliva testing into our infidelity detection testing in 2007 and are the only private company that includes saliva detection as part of every semen detection test.
  • Individuals needing a legal test, normally Attorneys representing a client, Private Investigators on surveillance, Forensic Companies, Police, and Government Laboratories, in anticipation of submission of the evidence in a court of law, normally require expert witness testimony. The following evidence submission requirements are necessary for your case to be treated as legal:
    • A general chain of custody or the transfer of the chain of custody
    • An exhibit submission form authorizing the test and return of evidence.
  • If you are interested in a legal test, contact us today at 844-441-8610.

We will review any semen detection report for free and provide suggestions. Email your report to [email protected]. Make sure you do more than just an AP home test which is meaningless alone. It is also very important not to do DNA testing alone. You need to have documented proof that the tested item contains semen, sperm, saliva, or blood.

AP tests are presumptive and a false positive or negative can occur. If you would like to confirm
the presence of semen, you may purchase our at home PSA kit here. You may also confirm your results by purchasing our laboratory semen, sperm, saliva detection testing here. This laboratory test can confirm the presence of sperm via microscopic examination. If it is remotely possible that the questionable stain is from your semen, perform a DNA confirmation test.

During destructive testing, the stained areas of the sample are cut so that the stain can be directly extracted. During non- destructive testing, the stained areas of the sample are swabbed, and the sample remains completely intact.

A positive saliva detection on male underwear can indicate oral sex with or without intercourse and a female DNA profile may be produced. A positive blood detection on male underwear can be due to indiscernible menstrual or vaginal bleeding from intercourse and may also produce a female DNA profile. A positive sperm detection indicates that ejaculation occurred as long as the contributor did not have a successful vasectomy or is not azoospermic. An odd location of fecal matter on the sample may produce two distinct male profiles after DNA testing.

AP (acid phosphatase) is an enzyme that occurs at a high concentration in semen. AP is commonly used as a presumptive test for the presence of semen. We do not use AP in our testing process as it is very common to get false negative and false positive results. Our statistics have demonstrated that 3% of all AP tests produce false positives and 35% produce false negatives. False positive results occur because AP is an enzyme that is present in cells hroughout the body and many other substances contain a high concentration of AP. False negative results occur because AP cannot be detected in samples containing less than 78 ng. Many AP tests on the market are mislabeled as an accurate and reliable test for semen but this is not a fact as all AP tests are presumptive and false negatives and positives are common. It is important to make sure you do not make any conclusions based solely off of a home AP test.