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Serology Accreditation Validation Study 1 - February 2008
EMAIL: Info@Semen-Detection.com for complete 10 page report
Purpose: To validate SSDI laboratory semen detections by performing three separate tests. The first two consist of chemical testing 20 vaginal swabs containing a controlled semen standard. Test number one is for Prostate Specific Antigen (PSA). PSA is a glycoprotein produced by the prostate gland and secreted in seminal fluid at concentrations (from 2.0x105 to 5.5x106 ng/ml). Test number two is to detect Acid Phosphatase (AP). AP is an enzyme present in semen at concentrations of 20 to 400 times other body fluids. The presence of AP is a presumptive test for the presence of semen. The third test is a physical microscopic examination for whole spermatozoa.
Twenty vaginal swabs (Seri part number R675D lot 0903114) with semen standard (Seri part number R563 lot 759) diluted to the following concentrations.
AP -Acid Phosphatase
PSA and AP Test Result Photographs
Conclusions: Prostate specific antigen can be detected from the undiluted range of 10,000 ng to a diluted range as low as 0.305175781 ng. Acid phosphatase can be detected from the undiluted range of 10,000 ng to a diluted range as low as 78.125 ng. The microscopic inspection was negative because there were no sperm heads contained in the semen standard.
Reproducibility and Precision: In order to determine reproducibility and precision, a positive control sample was tested ten times by PSA and AP in the following tests:
The reproducibility and precision of microscopic inspection is accomplished by confirming the presence of whole sperm heads mounted on a positive control sample Christmas tree stained slide (Kernechtrot-Picroindigocarmine Stain).